DNA Concentration Calculator
Calculate nucleic acid concentration from A260 absorbance, for double-stranded DNA, single-stranded DNA or RNA.
Check A260/A280 as well
Concentration alone says nothing about purity. A ratio of about 1.8 indicates clean DNA and 2.0 clean RNA; below 1.8 suggests protein or phenol contamination that will interfere with downstream enzymes. Quantification is only half the measurement.
What this page needs from you
This page asks for absorbance at 260 nm, type, dilution factor in × and sample volume in µL. From those it returns concentration in ng/µL, together with total yield, conversion factor used, in µg/ml and within the reliable range?. Every figure updates as you type, and nothing you enter leaves your browser.
Reproducibility depends on the inputs
The arithmetic here is exact; the measurements feeding it usually are not. Pipetting error, incomplete mixing and drift in a spectrophotometer all propagate straight through. Where a result matters, run it in duplicate and compare rather than trusting a single reading.
The formula
Concentration = A260 × conversion factor × dilution (50 ng/µL for dsDNA at A260 = 1)
Every result on this page comes from this formula. Nothing is rounded until the final display, so figures match a spreadsheet to the cent.
How to use the DNA Concentration Calculator
- Fill in each field with your own figures — the defaults are only there as an example.
- The result updates as you type, so there is no button to press.
- Check the breakdown under the main result for the supporting numbers.
- Use the copy button to take the result with you, or share the link to reload the same inputs.
DNA Concentration Calculator — frequently asked questions
Is this dna concentration calculator free to use?
Yes. Every calculator on the site is free, needs no sign-up, and runs entirely in your browser, so your figures never leave your device.
How accurate is the result?
The maths is exact for the formula shown on the page. Accuracy in the real world depends on your inputs and on assumptions the formula cannot see, so treat the output as a well-grounded estimate rather than a quote.
Can I use it on my phone?
Yes. The page is responsive, weighs a few kilobytes and works offline once loaded, so it behaves the same on a phone as on a desktop.
What people search for
This page answers each of these searches. They are the exact phrases visitors use to find the dna concentration calculator.
- dna concentration calculator
- dna yield calculator
- nucleic acid quantification
- nanodrop dna concentration
- a260 dna
About this calculator
Written and reviewed by The Samsung Calculator editorial team. Every calculator is written against a published formula, reviewed against at least one independent reference implementation, and dated when it changes.
Last reviewed September 14, 2026 · Calculation runs client-side · No data collected · Report an error